The Impacts of MDR1 Gene Polymorphism towards Plasma Rifampicin Levels in Javanese Pulmonary Tuberculosis
نویسندگان
چکیده
Rifampicin, one of the primary tuberculosis drugs, is a PGP substrate that is encoded by MDR 1 gene. The objectives of the research was to study the impacts of MDR1 gene polymorphism toward plasma rifampicin levels in Javanese patients with pulmonary tuberculosis. The subjects of this research are Javanese tuberculosis patients treated by fixed dosage combination (FDC) of anti-tuberculosis drugs containing rifampicin. There were 74 patients. The examination of MDR1 was conducted by employing restriction fragment length polymorphism (RFLP-PCR) method. Blood samplings to assays the rifampicin level were done on day 1; 4; 28; and 56. The measurement of rifampicin levels was done by high performance liquid chromatography (HPLC) method. There are significant difference on the level of plasma rifampicin between MDR1 CC, CT and TT with p <0,05. The polymorphism of MDR1 TT gene increase plasma rifampicin levels in Javanese patients with pulmonary tuberculosis.
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